Mouse Metabolism Core Services
Instruments within the Diabetes Center Metabolic Core include: a set of 12 metabolic chambers (Oxymax Lab Animal Monitoring System or CLAMS), a DEXA, a EchoMRI device and our new Seahorse Bioscience XFe24 Extracellular Flux Analyzer. The DEXA, EchoMRI, and Flux Analyzer are available for use, after training by any UCSF and, pending availability, non-UCSF laboratories. The CLAMS is not operated by Core users.
The UCSF Diabetes Center Metabolic Core operates four kinds of equipment:
To measure the metabolic rates from individual animal, we use a piece of equipment made by Columbus Instruments, Inc., called CLAMS (Columbus Instruments Comprehensive Lab Animal Monitoring System). Our CLAMS unit has 12 chambers. A typical CLAMS run takes 4 days and the machine can record metabolic data for up to 12 mice simultaneously. To improve the acclimation of the animals, we single-house the mice the week before the CLAMS run and we introduce them to powder food (used in a feeder in the center of the cage). They will still have access to pellets though. Make sure to transfer your animals in time if you are on a different campus.
We not analyze data for the user, we just format them (Excel file) so you have an average per hour for each metabolic parameter (VCO2, VO2, RER, food intake,...). However, I do provide extensive information and documentation on how to interpret the CLAMS data. Right now, those values are normalized to body weight (default output from the instrument).
Dual Energy X-ray Absorptiometry (DEXA) scanning is utilized to measure bone mineral density and tissue composition (fat mass & lean mass) in anesthetized mice. This device is housed in PS-644 (PS building). DEXA offers a relatively non-invasive method to measure body composition in small rodents, and it allows us to measure the body composition changes over time. The PIXImus DEXA scanner is ideal for longitudinal studies requiring repeated measurements on the same mouse. DEXA scans can be performed before and after compound administration to determine whether body composition has changed. Effects of genetic alterations, high fat feeding, surgical ovariectomy, etc. on body composition also can be measured.
Echo Magnetic Resonance Imaging (EchoMRI) from Echo Medical System, Houston, TX, is used to precisely measure whole body composition parameters such as total body fat, lean mass, body fluids, and total body water in live mice without the need for anesthesia or sedation. With its high sensitivity, EchoMRI is ideal for assessing small alterations in mouse whole body composition as a result of genetic mutation or exposure to novel compounds.
The Seahorse Bioscience XFe24 Extracellular Flux Analyzer is a platform for metabolic assays, simultaneously measuring the two major energy producing pathways of the cell – mitochondrial respiration and glycolysis - in a 24-well microplate, in real-time.
Contact us regarding training and if you are already user, remember to sign up using the Google calendar for the Seahorse.
CENTER MEMBERS ARE REMINDED TO ACKNOWLEDGE DRC CENTER GRANT (P30 DK063720) CONTRIBUTIONS WITH ‘THIS RESEARCH WAS SUPPORTED BY GRANTS X, Y FROM NIH P30 DK063720'.